Two specific markers for neural differentiation of embryonal carcinoma cells.
نویسندگان
چکیده
منابع مشابه
Dehydroepiandroesteron increased proliferation of neural progenitor cells derived from p19 embryonal carcinoma stem cells.
Introduction: The p19 line of embryonal carcinoma cells develops into neurons, astroglia and fibroblasts after aggregation and exposure to retinoic acid (RA). Dehydroepiandroesteron (DHEA) is a neurosteroid, can increase proliferation of human neural stem cell (NSC) and positively regulated the number of neurons produced. This study was initiated to assess the effect of DHEA on neural progenito...
متن کاملNeural differentiation of pluripotent mouse embryonal carcinoma cells by retinoic acid: inhibitory effect of serum.
In both embryonal carcinoma (EC) and embryonic stem (ES) cells, the differentiation pathway entered after treatment with retinoic acid (RA) varies as it is based upon different conditions of culture. This study employs mouse EC cells P19 to investigate the effects of serum on RA-induced neural differentiation occurring in a simplified monolayer culture. Cell morphology and expression of lineage...
متن کاملEnhanced Cardiomyogenic Differentiation of P19 Embryonal Carcinoma Stem Cells
BACKGROUND AND OBJECTIVES We investigated the effects of different concentrations of serum, 5-azacytidine, and culture time on the cardiomyogenic differentiation of P19 embryonal carcinoma stem cells in the course of developing an efficient protocol for generating the cardiomyogenic lineage. MATERIALS AND METHODS P19 cells were plated at a density of 1x10(6) cells on 10-cm bacterial dishes fo...
متن کاملDNA rearrangement activity during retinoic acid-induced neural differentiation of P19 mouse embryonal carcinoma cells.
Because of the many superficial similarities between the immune system and the central nervous system, it has long been speculated that somatic DNA recombination is, like the immune system, involved in brain development and function. To examine whether or not the V(D)J recombination signals of the immune system work in an in vitro neural differentiation model, the P19 mouse embryonal carcinoma ...
متن کاملRetinoic acid-induced neural differentiation of P19 embryonal carcinoma cells is potentiated by leukemia inhibitory factor.
Leukemia inhibitory factor (LIF) is a cytokine that exhibits proliferation, survival and differentiation in a wide range of cell types. Here we show that LIF potentiates retinoic acid-mediated neural induction in pluripotent P19 embryonal carcinoma cells. This activity of LIF was demonstrated by a profounded neural morphology followed by increased expression of neural-specific proteins (N-CAM, ...
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ژورنال
عنوان ژورنال: The EMBO Journal
سال: 1983
ISSN: 0261-4189
DOI: 10.1002/j.1460-2075.1983.tb01610.x